Device

Part:BBa_K1430006:Design

Designed by: Cedric Blötz   Group: iGEM14_Goettingen   (2014-09-30)


mRFP tag for yeast proteins


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2313
    Illegal BamHI site found at 2322
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 750
    Illegal AgeI site found at 2884
    Illegal AgeI site found at 2996
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Tool to select on LEU2 gene and tag proteins with mRFP.


Source

BBa_K530008 TDH3 Yeast Promoter

BBa_J04450 mRFP Coding Device

BBa_K801012 ADH1 yeast terminator

BBa_K165037 TEF2 yeast constitutive promoter

BBa_K530019 HHO1 Yeast UTR

BBa_I732007 BglII + BamHI

References